Lagrangian,

I think I did the last scale incorrectly. Here is another, uncompressed TIFF with the scale added using a much simpler and more accurate method: Uncompressed Image It's still been shrunken by Blogger. If you send me your email address, I can send you the full image via dropbox or something similar - it's over 28 MB in size.

Hi Clay,

Thanks! I sent you a private message with my e-mail.

Sincerely,
--Lagrangian

Hi Clay,

Thanks for the updated and uncompressed image.
I measured the 20 micron line to be 216 pixels long.

So a micron is about 10.8 pixels in length.

So the wavelengths of visible light (0.38 - 0.74 microns) would be 4 to 8 pixels.

The highest theoretical resolution for an optical microscope is about 1/2 wavelength, or 0.2 microns, which is around 2 pixels. Of course, most microscopes don't have such a high resolution in practice, but some get close.

Pretty neat!

Sincerely,
--Lagrangian

P.S. For the .jpg that Clay posted above, the 20 micron bar is about 97 pixels wide. So the pixel measurements are approximately one half the numbers above.